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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/08/2018 |
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Data da última atualização: |
12/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TOSCANO, J. H. B.; LOPES, L. G.; GIRALDELO, L. A.; SILVA, M. H. da; OKINO, C. H.; CHAGAS, A. C. de S. |
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Afiliação: |
João Henrique Barbosa Toscano, UNESP; Louyse Gabrielli Lopes, UNICEP; Luciana Aparecida Giraldelo, UNICEP; Matheus Henrique da Silva, UNICEP; CINTIA HIROMI OKINO, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.5, p.1253-1262, out. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. MenosDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes,... Mostrar Tudo |
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Palavras-Chave: |
Genes de referência. |
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Thesagro: |
Haemonchus Contortus; Ovino. |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02463naa a2200229 a 4500
001 2094611
005 2018-09-12
008 2018 bl uuuu u00u1 u #d
100 1 $aTOSCANO, J. H. B.
245 $aIdentification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.$h[electronic resource]
260 $c2018
520 $aDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results.
650 $aSheep
650 $aHaemonchus Contortus
650 $aOvino
653 $aGenes de referência
700 1 $aLOPES, L. G.
700 1 $aGIRALDELO, L. A.
700 1 $aSILVA, M. H. da
700 1 $aOKINO, C. H.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv.45, n.5, p.1253-1262, out. 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
11/07/2006 |
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Data da última atualização: |
16/09/2013 |
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Tipo da produção científica: |
Sistema de Produção |
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Autoria: |
PEREIRA, J. C. R. (Ed.). |
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Título: |
Cultura do guaranazeiro no Amazonas. |
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Edição: |
4. ed. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Manaus: Embrapa Amazônia Ocidental, 2005. |
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Páginas: |
40 p. il. color. |
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Série: |
(Embrapa Amazônia Ocidental. Sistemas de Produção, 2). |
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ISSN: |
1679-8880 |
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Idioma: |
Português |
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Notas: |
Autores: TAVARES, A. M.; ATROCH, A. L.; NASCIMENTO FILHO, F. J. do; PEREIRA, J. C. R.; ARAÚJO, J. C. A. de; MORAES, L. A. C.; SANTOS, L. P.; GARCIA, M. V. B.; ARRUDA, M. R. de; SOUSA, N. R.; ANGELO, P. C. da S. |
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Conteúdo: |
Cultura do Guaranazeiro no Amazonas; propagação do guaranazeiro; produção de mudas; implantação; tratos culturais; podas; colheita; despolpamento; torrefação; comercialização. |
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Palavras-Chave: |
Amazonas; Brasil; Cultivo; Cultura; Guaranazeiro; Paulinia cupana; Produção de muda; Propagação. |
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Thesagro: |
Colheita; Comercialização; Despolpamento; Guaraná; Muda; Paullinia Cupana; Poda; Pratica Cultural; Propagação Vegetativa; Sistema de Cultivo; Torrefação. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAA-2009-09/14953/1/Sistema_Prod_Guarana.pdf
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Marc: |
LEADER 01407nam a2200397 a 4500
001 1678408
005 2013-09-16
008 2005 bl uuuu u0uu1 u #d
022 $a1679-8880
100 1 $aPEREIRA, J. C. R. (Ed.).
245 $aCultura do guaranazeiro no Amazonas.
250 $a4. ed.
260 $aManaus: Embrapa Amazônia Ocidental$c2005
300 $a40 p. il. color.
490 $a(Embrapa Amazônia Ocidental. Sistemas de Produção, 2).
500 $aAutores: TAVARES, A. M.; ATROCH, A. L.; NASCIMENTO FILHO, F. J. do; PEREIRA, J. C. R.; ARAÚJO, J. C. A. de; MORAES, L. A. C.; SANTOS, L. P.; GARCIA, M. V. B.; ARRUDA, M. R. de; SOUSA, N. R.; ANGELO, P. C. da S.
520 $aCultura do Guaranazeiro no Amazonas; propagação do guaranazeiro; produção de mudas; implantação; tratos culturais; podas; colheita; despolpamento; torrefação; comercialização.
650 $aColheita
650 $aComercialização
650 $aDespolpamento
650 $aGuaraná
650 $aMuda
650 $aPaullinia Cupana
650 $aPoda
650 $aPratica Cultural
650 $aPropagação Vegetativa
650 $aSistema de Cultivo
650 $aTorrefação
653 $aAmazonas
653 $aBrasil
653 $aCultivo
653 $aCultura
653 $aGuaranazeiro
653 $aPaulinia cupana
653 $aProdução de muda
653 $aPropagação
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